Application

model to study effects of RA on chondrocytes, investigate differences between normal and pathological joints and evaluate potential treatment strategies

Cell Line Origin

Cartilage

Components

Basal Medium containing 10% FBS & 10% DMSO

General description

HC-RA Human Chondrocytes are derived from human articular cartilage of donors with rheumatoid arthritis (RA). Rheumatoid arthritis is an inflammatory disease that is characterized by overproliferation of synovial cells and their infiltration into adjacent joint tissues, accompanied by gradual destruction of cartilage and bone erosion. Normally, chondrocytes are responsible for producing and maintaining the extracellular matrix of cartilage, but in RA cartilage, the environment is changed significantly due to inflammation and the invasion of other cells. Therefore, HC-RA can be a useful model to study the effects of those changes on chondrocyte biology. In parallel with normal HC, HC-RA can be used to investigate the differences between normal and pathological joints and evaluate potential treatment strategies.Characterization: Positive for aggrecan after differentiation.

Preparation Note

1st passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO Can be cultured at least 10 doublings

Subculture Routine

Please refer to the HC-RA Culture Protocol.